Recombinant human Tryptase alpha/beta-1 protein
TPSAB1, also known as tryptase alpha/beta-1, is a serine protease with trypsin-like specificity. It is the key neutral protease present in mast cells and is discharged upon the coupled activation-degranulation response of this cell type. This protein is enzymatically active only as a heparin-stabilized tetramer, and is resistant to all known endogenous proteinase inhibitors. Also, it is implicated as a mediator in the pathogenesis of asthma and other allergic and inflammatory disorders. Recombinant human TPSAB1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Tryptase alpha/beta-1, TPSAB1, TPS1, TPS2, TPSB1
Expression System
Baculovirus
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Molecular Weight
28.2 kDa (251aa)
Additionnal Information
References & Citations
Schwartz, L.B., et al, (1985) J. Immunol. 135:2762-2767.; ; Lewicki L., et al, (2015) Mediators Inflamm. 2015:395173.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol
Scientific Category
Immunology
NCBI Accession Number
NP_003285.2
Uniprot Accession Number
Q15661
Species
Human
N Terminal Sequence
Ile31
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