Recombinant mouse GRP78/HSPA5 protein

HSPA5, also known as 78 kDa glucose-regulated protein precursor, is a member of the heat shock protein 70 (HSP70) family. It is localized in the lumen of the endoplasmic reticulum (ER), and is involved in the folding and assembly of proteins in the ER. As this protein interacts with many ER proteins, it may play a key role in monitoring protein transport through the cell. Recombinant mouse HSPA5 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Heat shock protein family A member 5, Heat shock 70kD protein 5, HSP70 family protein 5, Glucose-regulated protein 78kD, Binding-immunoglobulin protein, BiP, Endoplasmic reticulum chaperone BiP, Glucose-regulated protein 78kDa, Immunoglobulin heavy chain-binding protein

Expression System

E.coli

Antigen Species

Mouse

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by absorbance at 280 nm)

Purity

> 90% by SDS-PAGE

Molecular Weight

72.9 kDa (659aa) Confirmed by MALDI-TOF

Additionnal Information

Hspa5, 78 kDa glucose-regulated protein precursor, AL022860, AU019543, baffled, Bip, D2Wsu141e, D2Wsu17e, Grp78, Hsce70, mBiP, SEZ-7, Sez7, ATGP3685-10 µg, ATGP3685-20 µg, ATGP3685-50 µg, ATGP3685-100 µg, ATGP3685-250 µg, ATGP3685-500 µg, ATGP3685-1 mg, ATGP3685-10, ATGP3685-20, ATGP3685-50, ATGP3685-100, ATGP3685-250, ATGP3685-500, ATGP3685-1

References & Citations

Wang Q., et al. (2012) . Int J Oncol. 41 (2) :652-60.; ; Sokolowska I., et al. (2012) . FEBS J. 279 (14) :2579-94.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Heat Shock Proteins

NCBI Accession Number

NP_071705

Uniprot Accession Number

P20029

Species

Mouse

AA Sequence

EEEDKKE DVGTVVGIDL GTTYSCVGVF KNGRVEIIAN DQGNRITPSY VAFTPEGERL IGDAAKNQLT SNPENTVFDA KRLIGRTWND PSVQQDIKFL PFKVVEKKTK PYIQVDIGGG QTKTFAPEEI SAMVLTKMKE TAEAYLGKKV THAVVTVPAY FNDAQRQATK DAGTIAGLNV MRIINEPTAA AIAYGLDKRE GEKNILVFDL GGGTFDVSLL TIDNGVFEVV ATNGDTHLGG EDFDQRVMEH FIKLYKKKTG KDVRKDNRAV QKLRREVEKA KRALSSQHQA RIEIESFFEG EDFSETLTRA KFEELNMDLF RSTMKPVQKV LEDSDLKKSD IDEIVLVGGS TRIPKIQQLV KEFFNGKEPS RGINPDEAVA YGAAVQAGVL SGDQDTGDLV LLDVCPLTLG IETVGGVMTK LIPRNTVVPT KKSQIFSTAS DNQPTVTIKV YEGERPLTKD NHLLGTFDLT GIPPAPRGVP QIEVTFEIDV NGILRVTAED KGTGNKNKIT ITNDQNRLTP EEIERMVNDA EKFAEEDKKL KERIDTRNEL ESYAYSLKNQ IGDKEKLGGK LSSEDKETME KAVEEKIEWL ESHQDADIED FKAKKKELEE IVQPIISKLY GSGGPPPTGE EDTSEKDEL