Recombinant human Nicotinamide N-Methyltransferase/NNMT protein

NNMT (Nicotinamide N-methyltransferase) belongs to the family of transferases, specifically those transferring one-carbon group methyltransferases. It is predominantly expressed in the liver, and a lower expression is seen in the kidney, lung, skeletal muscle, placenta and heart. NNMT catalyzes the N-methylation of nicotinamide and other pyridines to form pyridinium ions. This activity is important for biotransformation of many drugs and xenobiotic compounds. Recombinant human NNMT protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Specifications

Specific activity is > 100 nmol/min/mg, and is defined as the amount of enzyme that transfer 1.0 nmole of methyl group per minute at 37C.

Product Name Alternative

Nicotinamide N-methyltransferase

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

Enzyme Activity, SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Bioactivity

Specific activity is > 100 nmol/min/mg, and is defined as the amount of enzyme that transfer 1.0 nmole of methyl group per minute at 37C.

Molecular Weight

37.7 kDa (284aa) confirmed by MALDI-TOF

Additionnal Information

Nicotinamide N-Methyltransferase, NNMT, Nicotinamide N-methyltransferase, ATGP3681-10 µg, ATGP3681-20 µg, ATGP3681-50 µg, ATGP3681-100 µg, ATGP3681-250 µg, ATGP3681-500 µg, ATGP3681-1 mg, ATGP3681-10, ATGP3681-20, ATGP3681-50, ATGP3681-100, ATGP3681-250, ATGP3681-500, ATGP3681-1

References & Citations

Parsons RB., et al. (2002) . J Neuropathol Exp Neurol. 61 (2) :111-24.; ; Smith ML., et al. (1998) . Biochim Biophys Acta. 1442 (2-3) :238-44.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_006160

Uniprot Accession Number

P40261

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MESGFTSKDT YLSHFNPRDY LEKYYKFGSR HSAESQILKH LLKNLFKIFC LDGVKGDLLI DIGSGPTIYQ LLSACESFKE IVVTDYSDQN LQELEKWLKK EPEAFDWSPV VTYVCDLEGN RVKGPEKEEK LRQAVKQVLK CDVTQSQPLG AVPLPPADCV LSTLCLDAAC PDLPTYCRAL RNLGSLLKPG GFLVIMDALK SSYYMIGEQK FSSLPLGREA VEAAVKEAGY TIEWFEVISQ SYSSTMANNE GLFSLVARKL SRPL