Recombinant human Glycine N-methyltransferase/GNMT protein
Glycine N-methyltransferase, also known as GNMT, catalyzes the synthesis of N-methylglycine (sarcosine) from glycine using S-adenosylmethionine (AdoMet) as the methyl donor. This protein affects DNA methylation by regulating the ratio of S-adenosylmethionine to S-adenosylhomocystine and participates in the detoxification pathway in liver cells. Also it is reported that GNMT expression is diminished in human hepatocellular carcinoma (HCC) . Recombinant human GNMT protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Specific activity is > 100 nmol/min/mg, and is defined as the amount of enzyme that transfer 1.0 nmole of methyl group per minute at 37C.
Product Name Alternative
Glycine N-methyltransferase
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Enzyme Activity
Concentration
1 mg/mL (determined by Bradford assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 95% by SDS-PAGE
Bioactivity
Specific activity is > 100 nmol/min/mg, and is defined as the amount of enzyme that transfer 1.0 nmole of methyl group per minute at 37C.
Molecular Weight
34.9 kDa (315aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Liao YJ., et al. (2009) Int J Cancer.124 (4) :816-26.; ; Huang YC., et al. (2008) J Gastroenterol Hepatol. 23 (9) :1384-9.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_061833
Uniprot Accession Number
Q14749
Species
Human
AA Sequence
Frequently Asked Questions
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