Recombinant human Glycine N-methyltransferase/GNMT protein

Glycine N-methyltransferase, also known as GNMT, catalyzes the synthesis of N-methylglycine (sarcosine) from glycine using S-adenosylmethionine (AdoMet) as the methyl donor. This protein affects DNA methylation by regulating the ratio of S-adenosylmethionine to S-adenosylhomocystine and participates in the detoxification pathway in liver cells. Also it is reported that GNMT expression is diminished in human hepatocellular carcinoma (HCC) . Recombinant human GNMT protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 100 nmol/min/mg, and is defined as the amount of enzyme that transfer 1.0 nmole of methyl group per minute at 37C.

Product Name Alternative

Glycine N-methyltransferase

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

1 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Bioactivity

Specific activity is > 100 nmol/min/mg, and is defined as the amount of enzyme that transfer 1.0 nmole of methyl group per minute at 37C.

Molecular Weight

34.9 kDa (315aa) confirmed by MALDI-TOF

Additionnal Information

Glycine N-methyltransferase, GNMT, Glycine N-methyltransferase, ATGP3679-10 µg, ATGP3679-20 µg, ATGP3679-50 µg, ATGP3679-100 µg, ATGP3679-250 µg, ATGP3679-500 µg, ATGP3679-1 mg, ATGP3679-10, ATGP3679-20, ATGP3679-50, ATGP3679-100, ATGP3679-250, ATGP3679-500, ATGP3679-1

References & Citations

Liao YJ., et al. (2009) Int J Cancer.124 (4) :816-26.; ; Huang YC., et al. (2008) J Gastroenterol Hepatol. 23 (9) :1384-9.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_061833

Uniprot Accession Number

Q14749

Species

Human

AA Sequence

MVDSVYRTRS LGVAAEGLPD QYADGEAARV WQLYIGDTRS RTAEYKAWLL GLLRQHGCQR VLDVACGTGV DSIMLVEEGF SVTSVDASDK MLKYALKERW NRRHEPAFDK WVIEEANWMT LDKDVPQSAE GGFDAVICLG NSFAHLPDCK GDQSEHRLAL KNIASMVRAG GLLVIDHRNY DHILSTGCAP PGKNIYYKSD LTKDVTTSVL IVNNKAHMVT LDYTVQVPGA GQDGSPGLSK FRLSYYPHCL ASFTELLQAA FGGKCQHSVL GDFKPYKPGQ TYIPCYFIHV LKRTD