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Recombinant human CD1d protein

CD1D, also known as antigen-presenting glycoprotein CD1d isoform 1, is a transmembrane glycoprotein in the CD1 family of glycolipid antigen-presenting MHC-like molecules. CD1d-presented lipid antigens activate a special class of T cells, known as natural killer T (NKT) cells, through the interaction with the T-cell receptor present on NKT membranes. When activated, NKT cells rapidly produce Th1 and Th2 cytokines, typically represented by interferon-gamma and interleukin 4 production. Recombinant human CD1D protein, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Antigen-presenting glycoprotein CD1d isoform 1, CD1D, CD1A, R3, R3G1

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 85% by SDS-PAGE

Molecular Weight

32.9 kDa (290aa)

Additionnal Information

CD1d, Antigen-presenting glycoprotein CD1d isoform 1, CD1D, CD1A, R3, R3G1, ATGP3678-10 µg, ATGP3678-20 µg, ATGP3678-50 µg, ATGP3678-100 µg, ATGP3678-250 µg, ATGP3678-500 µg, ATGP3678-1 mg, ATGP3678-10, ATGP3678-20, ATGP3678-50, ATGP3678-100, ATGP3678-250, ATGP3678-500, ATGP3678-1

References & Citations

Broxmeyer HE., et al, (2012) Blood. 119:5731-5741.; ; Huh JY., et al, (2017) Diabetes. 66:835-847.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Immunology

NCBI Accession Number

NP_001757.1

Uniprot Accession Number

P15813

Species

Human

N Terminal Sequence

Glu20

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