Recombinant human CD16b/FCGR3B protein
FCGR3B, also known as low affinity immunoglobulin gamma Fc region receptor III-B isoform 2, is a member of Ig super family. It encodes a glycosylphosphatidylinositol (GPI) -anchored protein that is expressed constitutively by neutrophils and after gamma-interferon stimulation by eosinophils. This protein binds to complex or aggregated IgG and monomeric IgG. Unlike III-A, it cannot mediate antibody-dependent cytotoxicity and phagocytosis. It can be a trap of immune complexes in peripheral blood circulation that does not activate neutrophils. Recombinant human FCGR3B, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Expression System
Baculovirus
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Molecular Weight
21.4 kDa (188aa)
Additionnal Information
References & Citations
Ravetch J.V., et al. (2001) Annu. Rev. Immunol. 19:275-290.; ; Machado LR., et al. (2012) Am. J. Hum. Genet. 90:973-985.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Immunology
NCBI Accession Number
NP_000561.3
Uniprot Accession Number
O75015
Species
Human
N Terminal Sequence
Glu21
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