Recombinant human IL-15R alpha/IL15RA protein
IL15RA, also known as interleukin-15 receptor subunit alpha isoform 1, is a high-affinity receptor for interleukin-15. It associates as a heterotrimer with the IL-2 receptor beta and gamma subunits to initiate signal transduction. This protein can signal both in cis and trans where IL15R from one subset of cells presents IL15 to neighboring IL2RG-expressing cells. IL15/IL15R alpha complexes can transmit reverse signaling that promotes cellular adhesion, tyrosine phosphorylation of intracellular proteins, and cytokine secretion by the IL 15/IL15R alpha expressing cells. Recombinant human IL15RA protein, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Human IL-15 R alpha inhibits Human IL-15 induced cell proliferation in the CTLL2 mouse cytotoxic T cells. The ED50 range ≤2 ng/mL
Product Name Alternative
Interleukin-15 receptor subunit alpha isoform 1, IL15RA, CD215, IL-15 receptor subunit alpha
Expression System
Baculovirus
Antigen Species
Human
Tag
HIgG-His-Tag
Applications
SDS-PAGE, Bioactivity
Concentration
0.5 mg/mL (determined by absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Bioactivity
Measured by its ability to inhibit proliferation using CTLL2 mouse cytotoxic T cells. The ED50 for this effect is ≤2 ng/mL with Human IL-15.
Molecular Weight
45.6 kDa (417aa)
Additionnal Information
References & Citations
Epardaud M., et al, (2008) Cancer Res. 68:2972-2983.; ; Stoklasek TA., et al, (2006) J Immunol. 177:6072-6080.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Apoptosis
NCBI Accession Number
NP_002180
Uniprot Accession Number
Q13261
Species
Human
N Terminal Sequence
Ala
AA Sequence
Frequently Asked Questions
Explore Other Products
Browse additional items from our catalog
CD66, pan (CEA) (Cocktail), CF647 conjugate, 0.1mg/mL
