Recombinant human BIN2 protein

BIN2, also known as bridging integrator 2 isoform1, is a cytoplasmic protein. It contains one BAR domain and Interacts with BIN1. This protein is highly expressed in some hematopoietic tissues, including peripheral blood, thymus, colon and placenta. It Promotes cell motility and migration, probably via its interaction with the cell membrane and with podosome proteins that mediate interaction with the cytoskeleton and also modulates membrane curvature and mediates membrane tubulation. Recombinant human BIN2 protein, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Bridging integrator 2 isoform1, BIN2, BRAP-1

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 80% by SDS-PAGE

Molecular Weight

66.1 kDa (606aa)

Additionnal Information

BIN2, Bridging integrator 2 isoform1, BIN2, BRAP-1, ATGP3671-10 µg, ATGP3671-20 µg, ATGP3671-50 µg, ATGP3671-100 µg, ATGP3671-250 µg, ATGP3671-500 µg, ATGP3671-1 mg, ATGP3671-10, ATGP3671-20, ATGP3671-50, ATGP3671-100, ATGP3671-250, ATGP3671-500, ATGP3671-1

References & Citations

Sanchez-Barrena MJ., et al, (2012) PLoS One. 7:e52401.; ; Hao Y., et al, (2016) Proc Natl Acad Sci U S A. 113:10418-10423.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol, 1mM DTT, 0.1mM PMSF.

Scientific Category

Cell Cycle

NCBI Accession Number

NP_057377

Uniprot Accession Number

Q9UBW5

Species

Human

N Terminal Sequence

Ala

AA Sequence

ADPMRGMPGA RTSSSGASEN HRARGQGGGP QGVGRMAEGK AGGAAGLFAK QVQKKFSRAQ EKVLQKLGKA VETKDERFEQ SASNFYQQQA EGHKLYKDLK NFLSAVKVMH ESSKRVSETL QEIYSSEWDG HEELKAIVWN NDLLWEDYEE KLADQAVRTM EIYVAQFSEI KERIAKRGRK LVDYDSARHH LEAVQNAKKK DEAKTAKAEE EFNKAQTVFE DLNQELLEEL PILYNSRIGC YVTIFQNISN LRDVFYREMS KLNHNLYEVM SKLEKQHSNK VFVVKGLSSS SRRSLVISPP VRTATVSSPL TSPTSPSTLS LKSESESVSA TEDLAPDAAQ GEDNSEIKEL LEEEEIEKEG SEASSSEEDE PLPACNGPAQ AQPSPTTERA KSQEEVLPSS TTPSPGGALS PSGQPSSSAT EVVLRTRTAS EGSEQPKKRA SIQRTSAPPS RPPPPRATAS PRPSSGNIPS SPTASGGGSP TSPRASLGTG TASPRTSLEV SPNPEPPEKP VRTPEAKENE NIHNQNPEEL CTSPTLMTSQ VASEPGEAKK MEDKEKDNKL ISANSSEGQD QLQVSMVPEN NNLTAPEPQE EVSTSENPQL HHHHHH