Recombinant mouse Crystallin gamma D/CRYGD protein
Crygd, also known as gamma-crystalin D, belongs to the beta/gamma-crystallin family. Crystallins are the dominant structural components of the vertebrate eye lens. Mammalian lens crystallins are divided into alpha, beta, and gamma families. Gamma-crystallins have been involved in cataract formation. Defects in Crygd are a cause of cataract autosomal dominant (ADC), cataract congenital non-nuclear polymorphic autosomal dominant (CCP), cataract congenital cerulean type 3 (CCA3) and cataract crystalline aculeiform (CACA) . Recombinant mouse Crygd protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Gamma-crystallin D, Aey4, Cryg-1, DGcry-1, Lop12
Expression System
E.coli
Antigen Species
Mouse
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by absorbance at 280 nm)
Purity
> 95% by SDS-PAGE
Molecular Weight
23.5 kDa (197aa) Confirmed by MALDI-TOF
Additionnal Information
References & Citations
Pande A., et al. (2001) Proc. Natl. Acad. Sci. U.S.A. 98 (11) :6116-20; ; Plotnikova O.V., et al. (2007) Am. J. Hum. Genet. 81 (1) :32-43
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol
Scientific Category
Neuroscience
NCBI Accession Number
NP_031802
Uniprot Accession Number
P04342
Species
Mouse
AA Sequence
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