Recombinant mouse LC3B/MAP1LC3B protein

Map1lc3b, also known as Microtubule-associated proteins 1A/1B light chain 3B, belongs to the MAP1 LC3 family. It is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Map1lc3b may also be involved in formation of autophagosomal vacuoles. It is expressed primarily in heart, testis, brain and skeletal muscle. Recombinant mouse Map1lc3b protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Microtubule-associated protein 1 light chain 3 beta, Microtubule-associated proteins 1A/1B light chain 3B, Autophagy-related protein LC3 B, Autophagy-related ubiquitin-like modifier LC3 B, MAP1 light chain 3-like protein 2, MAP1A/MAP1B light chain 3 B, MAP1A/MAP1B LC3 B, Atg8, LC3b, Map1lc3, Map1alc3

Expression System

E.coli

Antigen Species

Mouse

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by absorbance at 280 nm)

Purity

> 95% by SDS-PAGE

Molecular Weight

16.7 kDa (144aa) Confirmed by MALDI-TOF

Additionnal Information

Microtubule-associated proteins 1A/1B light chain 3B, Microtubule-associated protein 1 light chain 3 beta, Microtubule associated protein 1 light chain 3 beta, Map1lc3b, Map1lc3, Map1alc3, MAP1A/MAP1B light chain 3 B, MAP1A/MAP1B LC3 B, MAP1 light chain 3-like protein 2, LC3b, Autophagy-related ubiquitin-like modifier LC3 B, Autophagy-related protein LC3 B, Atg8, 1010001C15Rik, ATGP3668-10 µg, ATGP3668-20 µg, ATGP3668-50 µg, ATGP3668-100 µg, ATGP3668-250 µg, ATGP3668-500 µg, ATGP3668-1 mg, ATGP3668-010, ATGP3668-020, ATGP3668-050, ATGP3668-100, ATGP3668-250, ATGP3668-500, ATGP3668-01M

References & Citations

Behrends C., et al. (2010) Nature. 466 (7302) :68-76.; ; Yu S., et al. (2016) Tumour Biol. 37 (11) :15007-15017

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM MES buffer (pH 6.0) containing 0.1M NaCl, 1mM DTT, 30% glycerol

Scientific Category

Cytoskeleton

NCBI Accession Number

NP_080436

Uniprot Accession Number

Q9CQV6

Species

Mouse

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSHMPSEKT FKQRRSFEQR VEDVRLIREQ HPTKIPVIIE RYKGEKQLPV LDKTKFLVPD HVNMSELIKI IRRRLQLNAN QAFFLLVNGH SMVSVSTPIS EVYESERDED GFLYMVYASQ ETFG