Recombinant human DDR1 protein

DDR1, also known as epithelial discoidin domain-containing receptor 1 isoform 2, is a transmembrane glycoprotein that belongs to the discoidin-like domain containing subfamily of receptor tyrosine kinases. Expression of this protein is restricted to epithelial cells, particularly in the kidney, lung, gastrointestinal tract, and brain. In addition, it has been shown to be significantly overexpressed in several human tumors. DDR1 oligomerization enhances collagen binding and also modulates collagen fibrillogenesis. Recombinant human DDR1 protein, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Epithelial discoidin domain-containing receptor 1 isoform 2, DDR1, CAK, CD167, DDR, EDDR1, HGK2, MCK10, NEP, NTRK4, PTK3, PTK3A, RTK6, TRKE

Expression System

Baculovirus

Antigen Species

Human

Tag

HIgG-His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Molecular Weight

71kDa (636aa)

Additionnal Information

DDR1, Epithelial discoidin domain-containing receptor 1 isoform 2, DDR1, CAK, CD167, DDR, EDDR1, HGK2, MCK10, NEP, NTRK4, PTK3, PTK3A, RTK6, TRKE, ATGP3667-10 µg, ATGP3667-20 µg, ATGP3667-50 µg, ATGP3667-100 µg, ATGP3667-250 µg, ATGP3667-500 µg, ATGP3667-1 mg, ATGP3667-10, ATGP3667-20, ATGP3667-50, ATGP3667-100, ATGP3667-250, ATGP3667-500, ATGP3667-1

References & Citations

Ambrogio C., et al, (2016) Nat Med. 22:270-277.; ; Song J., et al, (2016) Tumour Biol. 37:11509-11521.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Neuroscience

NCBI Accession Number

NP_054699

Uniprot Accession Number

Q08345

Species

Human

N Terminal Sequence

Asp21

AA Sequence

DMKGHFDPAK CRYALGMQDR TIPDSDISAS SSWSDSTAAR HSRLESSDGD GAWCPAGSVF PKEEEYLQVD LQRLHLVALV GTQGRHAGGL GKEFSRSYRL RYSRDGRRWM GWKDRWGQEV ISGNEDPEGV VLKDLGPPMV ARLVRFYPRA DRVMSVCLRV ELYGCLWRDG LLSYTAPVGQ TMYLSEAVYL NDSTYDGHTV GGLQYGGLGQ LADGVVGLDD FRKSQELRYV WPGYDYVGWS NHSFSSGYVE MEFEFDRLRA FQAMQVHCNN MHTLGARLPG GVECRFRRGP AMAWEGEPMR HNLGGNLGDP RARAVSVPLG GRVARFLQCR FLFAGPWLLF SEISFISDVV NNSSPALGGT FPPAPWWPPG PPPTNFSSLE LEPRGQQPVA KAEGSPTALE PKSCDKTHTC PPCPAPELLG GPSVFLFPPK PKDTLMISRT PEVTCVVVDV SHEDPEVKFN WYVDGVEVHN AKTKPREEQY NSTYRVVSVL TVLHQDWLNG KEYKCKVSNK ALPAPIEKTI SKAKGQPREP QVYTLPPSRD ELTKNQVSLT CLVKGFYPSD IAVEWESNGQ PENNYKTTPP VLDSDGSFFL YSKLTVDKSR WQQGNVFSCS VMHEALHNHY TQKSLSLSPG KHHHHHH