Recombinant mouse Guanine deaminase/GDA protein
Gda, also known as guanine deaminase, catalyzes the hydrolytic deamination of guanine, producing xanthine and ammonia. Studies in rat ortholog suggest this gene plays a role in microtubule assembly. Multiple transcript variants encoding different isoforms have been found for this gene. Recombinant mouse Gda, fused to His-tag at N-terminus, was expressed in E. coli and purified by conventional chromatography techniques.
Product Specifications
Specifications
Specific activity is > 4,000 pmol/min/ug, and is defined as the amount of enzyme that convert guanine to xanthine per minute at pH 8.0 at 37C.
Product Name Alternative
Guanine deaminase, AU015411, AW047581, GAH, Guanase, Guanine aminase, Guanine aminohydrolase
Expression System
E.coli
Antigen Species
Mouse
Tag
His-Tag
Applications
SDS-PAGE, Enzyme Activity
Concentration
0.5 mg/mL (determined by absorbance at 280 nm)
Purity
> 95% by SDS-PAGE
Bioactivity
Specific activity is > 4,000pmol/min/ug, and is defined as the amount of enzyme that convert guanine to xanthine per minute at pH 8.0 at 37C.
Molecular Weight
53.4 kDa (477aa) Confirmed by MALDI-TOF
Additionnal Information
References & Citations
Yuan G, et al. (1999) . J Biol Chem. 274 (12) :8175-80; ; Trautwein-Schult A, et al (2014) J Mol Microbiol Biotechnol. 24 (2) :67-81
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 1mM DTT, 10% glycerol
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
NP_034396
Uniprot Accession Number
Q9R111
Species
Mouse
AA Sequence
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