Recombinant human B7-1/CD80 protein
CD80, also known as T-lymphocyte activation antigen CD80, is a member of cell surface immunoglobulin superfamily. This protein can bind to either human or mouse CD28 and CTLA-4. It is expressed on activated B cells, activated T cells, and macrophages. The B7/CD28/CTLA4 pathway has the ability to both positively and negatively regulate immune responses. Therefore, it is considered a promising therapeutic target for autoimmune diseases and various carcinomas. Recombinant human CD80, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Human CD80 is coated at 1 µg/mL (100 ul/well) can bind CD28 (CAT# ATGP3603) in a Functional ELISA assay.
Product Name Alternative
Expression System
Baculovirus
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Bioactivity
Concentration
0.5 mg/mL (determined by absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 95% by SDS-PAGE
Bioactivity
Measured by its binding ability in a functional ELISA with Human CD28 (CAT# ATGP3603)
Molecular Weight
24.9 kDa (216aa)
Additionnal Information
References & Citations
Zang X., et al. (2007) Clin Cancer Res. 13:5271-5279.; ; Kakoulidou M., et al. (2007) Scand. J. Immunol. 66:529-537.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Immunology
NCBI Accession Number
NP_005182
Uniprot Accession Number
P33681
Species
Human
N Terminal Sequence
Val35
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