Recombinant human B7-1/CD80 protein

CD80, also known as T-lymphocyte activation antigen CD80, is a member of cell surface immunoglobulin superfamily. This protein can bind to either human or mouse CD28 and CTLA-4. It is expressed on activated B cells, activated T cells, and macrophages. The B7/CD28/CTLA4 pathway has the ability to both positively and negatively regulate immune responses. Therefore, it is considered a promising therapeutic target for autoimmune diseases and various carcinomas. Recombinant human CD80, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Human CD80 is coated at 1 µg/mL (100 ul/well) can bind CD28 (CAT# ATGP3603) in a Functional ELISA assay.

Product Name Alternative

Activation B7-1 antigen, B7, B7.1, B7-1, BB1, CD28LG, CD28LG1, CD80, CTLA-4 counter-receptor B7.1, FcalphaRI, LAB7, T-lymphocyte activation antigen CD80

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Bioactivity

Concentration

0.5 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Bioactivity

Measured by its binding ability in a functional ELISA with Human CD28 (CAT# ATGP3603)

Molecular Weight

24.9 kDa (216aa)

Additionnal Information

B71, CD80, T-lymphocyte activation antigen CD80, CD80, B7, B7-1, B7.1, BB1, CD28LG, CD28LG1, LAB7, ATGP3663-10 µg, ATGP3663-20 µg, ATGP3663-50 µg, ATGP3663-100 µg, ATGP3663-250 µg, ATGP3663-500 µg, ATGP3663-1 mg, ATGP3663-10, ATGP3663-20, ATGP3663-50, ATGP3663-100, ATGP3663-250, ATGP3663-500, ATGP3663-1