Recombinant human NKG2D/KLRK1 protein

KLRK1, also known as NKG2D ligand 4 isoform 1, is a type ll transmembrane glycoprotein having an extracellular lectin-like domain. This domain lacks the recognizable calcium-binding sites found in true C-type lectins and binds protein rather than carbohydrate ligands. It can send co-stimulatory signals to activate CD8 T cells. Also, it plays an important role in viral control. Cellular stress can induce ligands for KLRK1 which results in the cell susceptible to NK cell-mediated lysis. Recombinant human KLRK1, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Human ULBP-6/RAET1L (CAT# ATGP4023) is coated at 10 µg/mL (100 ul/well) can bind Human NKG2D. The ED50 range ≤ 1.5 µg/mL.

Product Name Alternative

Killer cell lectin like receptor K1, Killer cell lectin-like receptor subfamily K member 1, NKG2-D type II integral membrane protein, NK cell receptor D, NKG2-D-activating NK receptor, CD314, NKG2D, KLR, NKG2-D

Expression System

Baculovirus

Antigen Species

Human

Tag

HIgG-His-Tag

Applications

SDS-PAGE, Bioactivity

Concentration

0.5 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Bioactivity

Measured by its binding ability in a functional ELISA with Human ULBP-6/RAET1L (CAT# ATGP4023) . The ED50 range ≤ 1.5 µg/mL.

Molecular Weight

43.9 kDa (386aa)

Additionnal Information

NKG2D, NKG2-D type II integral membrane protein, KLRK1, CD314, D12S2489E, KLR, NKG2-D, NKG2D, ATGP3662-10 µg, ATGP3662-20 µg, ATGP3662-50 µg, ATGP3662-100 µg, ATGP3662-250 µg, ATGP3662-500 µg, ATGP3662-1 mg, ATGP3662-10, ATGP3662-20, ATGP3662-50, ATGP3662-100, ATGP3662-250, ATGP3662-500, ATGP3662-1

References & Citations

Bauer S., et al, (1999) Science 285:727-729. ; ; Raulet DH., (2003) Nat. Rev. Immunol. 3:781-790.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Immunology

NCBI Accession Number

NP_031386.2

Uniprot Accession Number

P26718

Species

Human

N Terminal Sequence

Ala

AA Sequence

IWSAVFL NSLFNQEVQI PLTESYCGPC PKNWICYKNN CYQFFDESKN WYESQASCMS QNASLLKVYS KEDQDLLKLV KSYHWMGLVH IPTNGSWQWE DGSILSPNLL TIIEMQKGDC ALYASSFKGY IENCSTPNTY ICMQRTV