Recombinant human NKG2D/KLRK1 protein
KLRK1, also known as NKG2D ligand 4 isoform 1, is a type ll transmembrane glycoprotein having an extracellular lectin-like domain. This domain lacks the recognizable calcium-binding sites found in true C-type lectins and binds protein rather than carbohydrate ligands. It can send co-stimulatory signals to activate CD8 T cells. Also, it plays an important role in viral control. Cellular stress can induce ligands for KLRK1 which results in the cell susceptible to NK cell-mediated lysis. Recombinant human KLRK1, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Human ULBP-6/RAET1L (CAT# ATGP4023) is coated at 10 µg/mL (100 ul/well) can bind Human NKG2D. The ED50 range ≤ 1.5 µg/mL.
Product Name Alternative
Expression System
Baculovirus
Antigen Species
Human
Tag
HIgG-His-Tag
Applications
SDS-PAGE, Bioactivity
Concentration
0.5 mg/mL (determined by absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 95% by SDS-PAGE
Bioactivity
Measured by its binding ability in a functional ELISA with Human ULBP-6/RAET1L (CAT# ATGP4023) . The ED50 range ≤ 1.5 µg/mL.
Molecular Weight
43.9 kDa (386aa)
Additionnal Information
References & Citations
Bauer S., et al, (1999) Science 285:727-729. ; ; Raulet DH., (2003) Nat. Rev. Immunol. 3:781-790.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Immunology
NCBI Accession Number
NP_031386.2
Uniprot Accession Number
P26718
Species
Human
N Terminal Sequence
Ala
AA Sequence
Frequently Asked Questions
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