Recombinant human UMPS protein
UMPS, also known as uridine 5'-monophosphate synthase, is a bifunctional enzyme catalyzing the last two steps of de novo pyrimidine biosynthesis. It is an indispensable component in this metabolic pathway and is a target for antimalarial and antitumor drugs. Its expression in colorectal carcinoma tissues is not correlated with the toxicities of 5-FU-based regimen, but It in the normal tissues can help predict the toxicities associated with 5-FU. Recombinant human UMPS, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Uridine 5'-monophosphate synthase, UMPS, OPRT
Expression System
Baculovirus
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 95% by SDS-PAGE
Molecular Weight
53 kDa (486aa)
Additionnal Information
References & Citations
Zhang Y., et al. (2013) J Biol Chem. 288:34746-34754.; ; Dong Q., et al. (2012) Nan Fang Yi Ke Da Xue Xue Bao. 32:1179-1181.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 30% glycerol
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
NP_000364.1
Uniprot Accession Number
P11172
Species
Human
N Terminal Sequence
Met1
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