Recombinant mouse Sepiapterin reductase/SPR protein

Spr also known as Sepiapterin reductase, belongs to the Short-chain dehydrogenase/reductase (Sdr) family and also reduces various exogenous carbonyl compounds including phenylpropanedione. Spr is an essential enzyme for the biosynthesis of tetrahydrobiopterin, an essential cofactor for aromatic amino acid hydrolases including tyrosine hydroxylase, the rate-limiting enzyme in dopamine synthesis. Defects in Spr cause DOPA-responsive dystonia defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. Recombinant mouse Spr protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Sepiapterin reductase, AA409688, Gm10328

Expression System

E.coli

Antigen Species

Mouse

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by absorbance at 280 nm)

Purity

> 95% by SDS-PAGE

Molecular Weight

30.3 kDa (285aa) confirmed by MALDI-TOF

Additionnal Information

Spr, Sepiapterin reductase, AA409688, Gm10328, ATGP3652-10 µg, ATGP3652-20 µg, ATGP3652-50 µg, ATGP3652-100 µg, ATGP3652-250 µg, ATGP3652-500 µg, ATGP3652-1 mg, ATGP3652-10, ATGP3652-20, ATGP3652-50, ATGP3652-100, ATGP3652-250, ATGP3652-500, ATGP3652-1

References & Citations

Tobin JE., et al. (2007) . Brain Res. 30; ;1139:42-7.; ; Ohye T., et al. (1998) . Biochem Biophys Res Commun. 251 (2) :597-602.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.5) containing 1mM DTT, 10% glycerol

Scientific Category

Redox Proteins

NCBI Accession Number

NP_035597

Uniprot Accession Number

Q91XH5

Species

Mouse

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMEAGGLG CAVCVLTGAS RGFGRALAPQ LARLLSPGSV MLVSARSESM LRQLKEELGA QQPDLKVVLA AADLGTEAGV QRLLSAVREL PRPEGLQRLL LINNAATLGD VSKGFLNVND LAEVNNYWAL NLTSMLCLTS GTLNAFQDSP GLSKTVVNIS SLCALQPYKG WGLYCAGKAA RDMLYQVLAA EEPSVRVLSY APGPLDNDMQ QLARETSKDP ELRSKLQKLK SDGALVDCGT SAQKLLGLLQ KDTFQSGAHV DFYDC