What is the catalog number for Recombinant mouse TNF-alpha protein?

The catalog number for Recombinant mouse TNF-alpha protein is ATGP3651-500.

What sizes are available for Recombinant mouse TNF-alpha protein?

Recombinant mouse TNF-alpha protein is available in the following size(s): 500 µg.

Is Recombinant mouse TNF-alpha protein in stock?

Recombinant mouse TNF-alpha protein is currently made to order / not held in immediate stock. Contact us for lead time.

Recombinant mouse TNF-alpha protein

TNFa, also known as tumor necrosis factor, binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia. Recombinant mouse TNFa protein was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Specifications

Mouse TNF-alpha induces cell cytotoxicity in the L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D.

Product Name Alternative

Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNFSF2, TNF-a, TNFA, TNF

Expression System

E.coli

Antigen Species

Mouse

Tag

Non-Tagged

Applications

SDS-PAGE, Bioactivity

Concentration

1 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Bioactivity

Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 range ≤ 0.06 ng/mL.

Molecular Weight

17.4 kDa (157aa) confirmed by MALDI-TOF

Additionnal Information

TNF-a, Tumor necrosis factor, DIF, TNF-alpha, TNFalpha, Tnfsf1a, TNFSF2, Cachectin, ATGP3738, ATGP3651-10 µg, ATGP3651-20 µg, ATGP3651-50 µg, ATGP3651-100 µg, ATGP3651-250 µg, ATGP3651-500 µg, ATGP3651-1 mg, ATGP3651-10, ATGP3651-20, ATGP3651-50, ATGP3651-100, ATGP3651-250, ATGP3651-500, ATGP3651-1, ATGP3651-10 µg, ATGP3651-20 µg, ATGP3651-50 µg, ATGP3651-100 µg, ATGP3651-250 µg, ATGP3651-500 µg, ATGP3651-1 mg, ATGP3651-10, ATGP3651-20, ATGP3651-50, ATGP3651-100, ATGP3651-250, ATGP3651-500, ATGP3651-1

References & Citations

Shakhov A.N., et al. (1987) Bioorg. Khim. 13:701-705; ; Pennica D., et al. (1985) Proc. Natl. Acad. Sci. U.S.A. 82:6060-6064

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4)

Scientific Category

Hormones & Cytokines

NCBI Accession Number

NP_038721

Uniprot Accession Number

P06804

Species

Mouse

AA Sequence

MLRSSSQNSS DKPVAHVVAN HQVEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLVY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK VNLLSAVKSP CPKDTPEGAE LKPWYEPIYL GGVFQLEKGD QLSAEVNLPK YLDFAESGQV YFGVIAL