Recombinant mouse Adenosylhomocysteinase/AHCY protein

Ahcy, also known as Adenosylhomocysteinase, is an enzyme that catalyzes the reversible hydrolysis of S-sdenosylhomocysteine (AdoHcy) to adenosine (Ado) and L-homocysteine (Hcy) . AdoHcy hydrolysis is a reversible reaction with an equilibrium favoring AdoHcy formation, but hydrolysis prevails under physiological conditions due to the rapid removal of adenosine and homocysteine. Thus, AHCY s activity in mammals is directly related to homocysteine level, an independent risk factor for vascular disease. It also functions as a regulator of biological transmethylation by controlling the concentration of AdoHcy, a potent competitive inhibitor of all S-adenosyl-L-methionine methyltransferases. Recombinant mouse Ahcy, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

S-adenosylhomocysteine hydrolase, AdoHcyase, CUBP, Liver copper-binding protein, S-adenosyl-L-homocysteine hydrolase, SAHH

Expression System

E.coli

Antigen Species

Mouse

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by absorbance at 280 nm)

Purity

> 90% by SDS-PAGE

Molecular Weight

50.2 kDa (456aa) confirmed by MALDI-TOF

Additionnal Information

Ahcy, Adenosylhomocysteinase, AA987153, AL024110, CuBP, SAHH, ATGP3650-10 µg, ATGP3650-20 µg, ATGP3650-50 µg, ATGP3650-100 µg, ATGP3650-250 µg, ATGP3650-500 µg, ATGP3650-1 mg, ATGP3650-10, ATGP3650-20, ATGP3650-50, ATGP3650-100, ATGP3650-250, ATGP3650-500, ATGP3650-1

References & Citations

Vugrek O., et al. (2009) Hum Mutat. 30 (4) : E555-65.; ; Park SJ. et al., (2015) Am J Cancer Res. 5 (7) :2127-2138

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 40% glycerol, 0.2M NaCl, 1mM DTT

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_057870

Uniprot Accession Number

P50247

Species

Mouse

AA Sequence

MSDKLP YKVADIGLAA WGRKALDIAE NEMPGLMRMR EMYSASKPLK GARIAGCLHM TVETAVLIET LVALGAEVRW SSCNIFSTQD HAAAAIAKAG IPVFAWKGET DEEYLWCIEQ TLHFKDGPLN MILDDGGDLT NLIHTKYPQL LSGIRGISEE TTTGVHNLYK MMSNGILKVP AINVNDSVTK SKFDNLYGCR ESLIDGIKRA TDVMIAGKVA VVAGYGDVGK GCAQALRGFG ARVIITEIDP INALQAAMEG YEVTTMDEAC KEGNIFVTTT GCVDIILGRH FEQMKDDAIV CNIGHFDVEI DVKWLNENAV EKVNIKPQVD RYWLKNGRRI ILLAEGRLVN LGCAMGHPSF VMSNSFTNQV MAQIELWTHP DKYPVGVHFL PKKLDEAVAE AHLGKLNVKL TKLTEKQAQY LGMPINGPFK PDHYRY