Recombinant human Frizzled-4 protein

FZD4, also known as frizzled-4, is a 7- transmembrane glycoprotein of the Frizzled family within the G-protein coupled receptor superfamily. This protein may play a role as a positive regulator of the Wingless type MMTV integration site signaling pathway. This pathways seem to involve interactions with G-proteins. It is involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Recombinant human FZD4, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Frizzled-4, FZD4, CD344, EVR1, FEVR, Fz-4, Fz4, FZD4S, FzE4, GPCR, hFz4

Expression System

Baculovirus

Antigen Species

Human

Tag

HIgG-His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Molecular Weight

48.3 kDa (428aa)

Additionnal Information

Frizzled4, Frizzled-4, FZD4, CD344, EVR1, FEVR, Fz-4, Fz4, FZD4S, FzE4, GPCR, hFz4, ATGP3645-10 µg, ATGP3645-20 µg, ATGP3645-50 µg, ATGP3645-100 µg, ATGP3645-250 µg, ATGP3645-500 µg, ATGP3645-1 mg, ATGP3645-10, ATGP3645-20, ATGP3645-50, ATGP3645-100, ATGP3645-250, ATGP3645-500, ATGP3645-1

References & Citations

Kirikoshi H., et al. (1999) Biochem Biophys Res Commun. 264:955-961.; ; Xu Q., et al. (2004) Cell. 116:883-895.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Signal Transduction

NCBI Accession Number

NP_036325

Uniprot Accession Number

Q9ULV1

Species

Human

N Terminal Sequence

Ala

AA Sequence

ADPFGDEEER RCDPIRISMC QNLGYNVTKM PNLVGHELQT DAELQLTTFT PLIQYGCSSQ LQFFLCSVYV PMCTEKINIP IGPCGGMCLS VKRRCEPVLK EFGFAWPESL NCSKFPPQND HNHMCMEGPG DEEVPLPHKT PIQPGEECHS VGTNSDQYIW VKRSLNCVLK CGYDAGLYSR SAKEFTDIWL EPKSCDKTHT CPPCPAPELL GGPSVFLFPP KPKDTLMISR TPEVTCVVVD VSHEDPEVKF NWYVDGVEVH NAKTKPREEQ YNSTYRVVSV LTVLHQDWLN GKEYKCKVSN KALPAPIEKT ISKAKGQPRE PQVYTLPPSR DELTKNQVSL TCLVKGFYPS DIAVEWESNG QPENNYKTTP PVLDSDGSFF LYSKLTVDKS RWQQGNVFSC SVMHEALHNH YTQKSLSLSP GKHHHHHH