Recombinant mouse Lactate Dehydrogenase B/LDHB protein

Ldhb, also known as L-lactate dehydrogenase B chain isoform Ldhb, is a member of the lactate dehydrogenase family. It is an oxidoreductase which catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. As this protein can also catalyze the oxidation of hydroxybutyrate, it is occasionally called Hydroxybutyrate Dehydrogenase (HBD) . The LDH family consists of three members, LDH-A, LDH-B and LDH-C. LDHs function as powerful markers for germ cell tumors. Recombinant mouse Ldhb protein, fused to His-tagging at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 150 units/mg, in which one unit will convert 1.0 umole of pyruvate to L-lactate and beta-NAD per minute at pH 7.5.

Product Name Alternative

L-lactate dehydrogenase B chain isoform Ldhb, AI790582, H-Ldh, Ldh-2, LDH-B, LDH-H, Ldh2, LDH heart subunit

Expression System

E.coli

Antigen Species

Mouse

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

1 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Bioactivity

Specific activity is > 150unit/mg, in which one unit will convert 1.0 umole of pyruvate to L-lactate and beta-NAD per minute at pH 7.5.

Molecular Weight

39 kDa (357aa) confirmed by MALDI-TOF

Additionnal Information

L-lactate dehydrogenase B chain, Ldhb, L-lactate dehydrogenase B chain isoform Ldhb, AI790582, H-Ldh, Ldh-2, LDH-B, LDH-H, Ldh2, ATGP3643-10 µg, ATGP3643-20 µg, ATGP3643-50 µg, ATGP3643-100 µg, ATGP3643-250 µg, ATGP3643-500 µg, ATGP3643-1 mg, ATGP3643-10, ATGP3643-20, ATGP3643-50, ATGP3643-100, ATGP3643-250, ATGP3643-500, ATGP3643-1

References & Citations

Read JA., et al. (2001) Proteins. 43 (2) :175-85.; ; Butt AA., et al. (2002) AIDS Read. 12 (7) :317-21

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

Scientific Category

Cancer

NCBI Accession Number

NP_032518

Uniprot Accession Number

P16125

Species

Mouse

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMATLKEK LIASVADDEA AVPNNKITVV GVGQVGMACA ISILGKSLAD ELALVDVLED KLKGEMMDLQ HGSLFLQTPK IVADKDYSVT ANSKIVVVTA GVRQQEGESR LNLVQRNVNV FKFIIPQIVK YSPDCTIIVV SNPVDILTYV TWKLSGLPKH RVIGSGCNLD SARFRYLMAE KLGIHPSSCH GWILGEHGDS SVAVWSGVNV AGVSLQELNP EMGTDNDSEN WKEVHKMVVD SAYEVIKLKG YTNWAIGLSV ADLIESMLKN LSRIHPVSTM VKGMYGIENE VFLSLPCILN ARGLTSVINQ KLKDDEVAQL RKSADTLWDI QKDLKDL