Recombinant human SLAMF2/CD48 protein
CD48, also known as CD48 antigen isoform 1, is a GPI-linked protein in the CD2 family of immunoglobulin superfamily molecules. This protein is competent to transduce signals and can also trigger signaling through CD2 or 2B4. CD48-CD2 interactions promote T cell activation and class switching to IgG2a in B cells. High affinity CD48-2B4 interactions can either promote or inhibit NK cell and cytotoxic T cell (CTL) activation. It expressed on NK cells is coactivating, whereas CD48 expressed on other cell types inhibits NK cell activation. Recombinant human CD48, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Human CD48/SLAMF2 is coated at 2 µg/mL (100 ul/well) can bind Human CD244 (CAT# ATGP3552) in a Functional ELISA assay.
Product Name Alternative
Expression System
Baculovirus
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Bioactivity
Concentration
1 mg/mL (determined by absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Bioactivity
Measured by its binding ability in a functional ELISA with Human CD244 (CAT# ATGP3552)
Molecular Weight
23.4 kDa (203aa)
Additionnal Information
References & Citations
Kis-Toth K., et al, (2014) J. Immunol. 192:4436-4442.; ; Fisher RC., et al, (1991) Mol. Cell. Biol. 11:1614-1623.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Immunology
NCBI Accession Number
NP_001769.2
Uniprot Accession Number
P09326
Species
Human
N Terminal Sequence
Ala
AA Sequence
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