Recombinant human SLAMF2/CD48 protein

CD48, also known as CD48 antigen isoform 1, is a GPI-linked protein in the CD2 family of immunoglobulin superfamily molecules. This protein is competent to transduce signals and can also trigger signaling through CD2 or 2B4. CD48-CD2 interactions promote T cell activation and class switching to IgG2a in B cells. High affinity CD48-2B4 interactions can either promote or inhibit NK cell and cytotoxic T cell (CTL) activation. It expressed on NK cells is coactivating, whereas CD48 expressed on other cell types inhibits NK cell activation. Recombinant human CD48, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Human CD48/SLAMF2 is coated at 2 µg/mL (100 ul/well) can bind Human CD244 (CAT# ATGP3552) in a Functional ELISA assay.

Product Name Alternative

B-cell membrane protein, B-lymphocyte activation marker BLAST-1, BCM1 surface antigen, Leukocyte antigen MEM-102, SLAM family member 2, Signaling lymphocytic activation molecule 2, TCT.1

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Bioactivity

Concentration

1 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Bioactivity

Measured by its binding ability in a functional ELISA with Human CD244 (CAT# ATGP3552)

Molecular Weight

23.4 kDa (203aa)

Additionnal Information

CD48, SLAMF2, CD48 antigen isoform 1, CD48, BCM1, BLAST, BLAST1, hCD48, mCD48, MEM-102, SLAMF2, ATGP3642-10 µg, ATGP3642-20 µg, ATGP3642-50 µg, ATGP3642-100 µg, ATGP3642-250 µg, ATGP3642-500 µg, ATGP3642-1 mg, ATGP3642-10, ATGP3642-20, ATGP3642-50, ATGP3642-100, ATGP3642-250, ATGP3642-500, ATGP3642-1

References & Citations

Kis-Toth K., et al, (2014) J. Immunol. 192:4436-4442.; ; Fisher RC., et al, (1991) Mol. Cell. Biol. 11:1614-1623.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Immunology

NCBI Accession Number

NP_001769.2

Uniprot Accession Number

P09326

Species

Human

N Terminal Sequence

Ala

AA Sequence

QGHLVHM TVVSGSNVTL NISESLPENY KQLTWFYTFD QKIVEWDSRK SKYFESKFKG RVRLDPQSGA LYISKVQKED NSTYIMRVLK KTGNEQEWKI KLQVLDPVPK PVIKIEKIED MDDNCYLKLS CVIPGESVNY TWYGDKRPFP KELQNSVLET TLMPHNYSRC YTCQVSNSVS SKNGTVCLSP PCTLARS