Recombinant human Siglec-7/CD328 protein

SIGLEC7, also known as sialic acid-binding Ig-like lectin 7 isoform 1, is a recently discovered family of sialic acid-binding lectins of the immunoglobulin (Ig) superfamily. The extracellular portion has two Ig-like domains, with the amino-terminal V-set Ig domain including amino acid residues known to be involved in sialic acid recognition by other Siglecs. The combination of an extracellular sialic acid binding site and an intracellular ITIM motif suggests that this molecule is involved in trans-membrane regulatory signaling reactions. It exists as a monomer on the cell surface and is expressed on natural killer cells. Recombinant human SIGLEC7, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Sialic acid-binding Ig-like lectin 7 isoform 1, SIGLEC7, AIRM1, CD328, CDw328, D-siglec, p75, p75/AIRM1, QA79, SIGLEC-7, SIGLEC19P, SIGLECP2

Expression System

Baculovirus

Antigen Species

Human

Tag

HIgG-His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Molecular Weight

64.2 kDa (577aa)

Additionnal Information

Siglec7, CD328, Sialic acid-binding Ig-like lectin 7 isoform 1, SIGLEC7, AIRM1, CD328, CDw328, D-siglec, p75, p75/AIRM1, QA79, SIGLEC-7, SIGLEC19P, SIGLECP2, ATGP3637-10 µg, ATGP3637-20 µg, ATGP3637-50 µg, ATGP3637-100 µg, ATGP3637-250 µg, ATGP3637-500 µg, ATGP3637-1 mg, ATGP3637-10, ATGP3637-20, ATGP3637-50, ATGP3637-100, ATGP3637-250, ATGP3637-500, ATGP3637-1

References & Citations

Crocker., et al. (2001) Trends Immunol. 22:337-342.; ; Nicoll G., et al. (1999) J. Biol. Chem. 274:34089-34095.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Cardiovascular

NCBI Accession Number

NP_055200

Uniprot Accession Number

Q9Y286

Species

Human

N Terminal Sequence

Ala

AA Sequence

QKSNRKD YSLTMQSSVT VQEGMCVHVR CSFSYPVDSQ TDSDPVHGYW FRAGNDISWK APVATNNPAW AVQEETRDRF HLLGDPQTKN CTLSIRDARM SDAGRYFFRM EKGNIKWNYK YDQLSVNVTA LTHRPNILIP GTLESGCFQN LTCSVPWACE QGTPPMISWM GTSVSPLHPS TTRSSVLTLI PQPQHHGTSL TCQVTLPGAG VTTNRTIQLN VSYPPQNLTV TVFQGEGTAS TALGNSSSLS VLEGQSLRLV CAVDSNPPAR LSWTWRSLTL YPSQPSNPLV LELQVHLGDE GEFTCRAQNS LGSQHVSLNL SLQQEYTGKM RPVSGVLL