Recombinant human N-Cadherin/CDH2 protein

CDH2, also known as cadherin-2 isoform 1, is a transmembrane, homophilic glycoprotein belonging to the calcium-dependent cell adhesion molecule family. It plays a role in neurons and later was found to also play a role in cardiac muscle and in cancer metastasis. Its loss promotes tumorigenesis by releasing membrane-bound B-catenin, hence stimulating Wnt signaling. It seems to be involved in tumor development, but this finding is limited in adrenocortical tumors (ACTs) . Recombinant human CDH2, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Cadherin-2 isoform 1, CDH2, CD325, CDHN, CDw325, NCAD, Neural cadherin

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Molecular Weight

62.9 kDa (574aa)

Additionnal Information

N-Cadherin, Cadherin-2 isoform 1, CDH2, CD325, CDHN, CDw325, NCAD, ATGP3634-10 µg, ATGP3634-20 µg, ATGP3634-50 µg, ATGP3634-100 µg, ATGP3634-250 µg, ATGP3634-500 µg, ATGP3634-1 mg, ATGP3634-10, ATGP3634-20, ATGP3634-50, ATGP3634-100, ATGP3634-250, ATGP3634-500, ATGP3634-1

References & Citations

Buxton RS., et al. (1992) Semin Cell Biol. 3:157-167.; ; Rubin B., et al. (2016) Tumour Biol. 37:13545-13555.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol

Scientific Category

Signal Transduction

NCBI Accession Number

NP_001783

Uniprot Accession Number

P19022

Species

Human

N Terminal Sequence

Ala

AA Sequence

ADPDWVIPPI NLPENSRGPF PQELVRIRSD RDKNLSLRYS VTGPGADQPP TGIFIINPIS GQLSVTKPLD REQIARFHLR AHAVDINGNQ VENPIDIVIN VIDMNDNRPE FLHQVWNGTV PEGSKPGTYV MTVTAIDADD PNALNGMLRY RIVSQAPSTP SPNMFTINNE TGDIITVAAG LDREKVQQYT LIIQATDMEG NPTYGLSNTA TAVITVTDVN DNPPEFTAMT FYGEVPENRV DIIVANLTVT DKDQPHTPAW NAVYRISGGD PTGRFAIQTD PNSNDGLVTV VKPIDFETNR MFVLTVAAEN QVPLAKGIQH PPQSTATVSV TVIDVNENPY FAPNPKIIRQ EEGLHAGTML TTFTAQDPDR YMQQNIRYTK LSDPANWLKI DPVNGQITTI AVLDRESPNV KNNIYNATFL ASDNGIPPMS GTGTLQIYLL DINDNAPQVL PQEAETCETP DPNSINITAL DYDIDPNAGP FAFDLPLSPV TIKRNWTITR LNGDFAQLNL KIKFLEAGIY EVPIIITDSG NPPKSNISIL RVKVCQCDSN GDCTDVDRIV GAGLGTGAHH HHHH