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Recombinant human CD277/BTN3A1 protein

BTN3A1, also known as butyrophilin subfamily 3 member A1 isoform, belongs to the immunoglobulin superfamily. It is composed of an extracellular N-terminal IgV and a membrane proximal IgC domain followed by a transmembrane domain and a cytoplasmic tail. This protein plays a role in T-cell activation and in the adaptive immune response. Also, it regulates the proliferation of activated T-cells and the release of cytokines and IFNG by activated T-cells. Recombinant human BTN3A1, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Butyrophilin subfamily 3 member A1 isoform, BTN3A1, BT3.1, BTF5, BTN3.1, CD277

Expression System

Baculovirus

Antigen Species

Human

Tag

HIgG-His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Molecular Weight

51.1 kDa (464aa)

Additionnal Information

CD277, BTN3A1, Butyrophilin subfamily 3 member A1 isoform, BTN3A1, BT3.1, BTF5, BTN3.1, CD277, ATGP3633-10 µg, ATGP3633-20 µg, ATGP3633-50 µg, ATGP3633-100 µg, ATGP3633-250 µg, ATGP3633-500 µg, ATGP3633-1 mg, ATGP3633-10, ATGP3633-20, ATGP3633-50, ATGP3633-100, ATGP3633-250, ATGP3633-500, ATGP3633-1

References & Citations

Rhodes DA., et al, (2015) J. Immunol. 194:2390-2398.; ; Sandstrom A., et al, (2014) Immunity 40:490-500.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Immunology

NCBI Accession Number

NP_008979.3

Uniprot Accession Number

O00481

Species

Human

N Terminal Sequence

Gln30

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