Recombinant human CD277/BTN3A1 protein
BTN3A1, also known as butyrophilin subfamily 3 member A1 isoform, belongs to the immunoglobulin superfamily. It is composed of an extracellular N-terminal IgV and a membrane proximal IgC domain followed by a transmembrane domain and a cytoplasmic tail. This protein plays a role in T-cell activation and in the adaptive immune response. Also, it regulates the proliferation of activated T-cells and the release of cytokines and IFNG by activated T-cells. Recombinant human BTN3A1, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Butyrophilin subfamily 3 member A1 isoform, BTN3A1, BT3.1, BTF5, BTN3.1, CD277
Expression System
Baculovirus
Antigen Species
Human
Tag
HIgG-His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Molecular Weight
51.1 kDa (464aa)
Additionnal Information
References & Citations
Rhodes DA., et al, (2015) J. Immunol. 194:2390-2398.; ; Sandstrom A., et al, (2014) Immunity 40:490-500.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Immunology
NCBI Accession Number
NP_008979.3
Uniprot Accession Number
O00481
Species
Human
N Terminal Sequence
Gln30
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