Recombinant human PD-L1/B7-H1 protein
CD274, also known as programmed cell death 1 ligand 1 isoform, is a member of the growing B7 family of immune molecules. This protein is involved in the regulation of cellular and humoral immune responses. It suppresses T cell activation and proliferation and induces the apoptosis of activated T cells. Also, it plays a role in the development of immune tolerance by promoting T cell anergy and enhancing regulatory T cell development. Recombinant human CD274, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Human PD-1 (CAT# ATGP4027) is coated at 5 µg/mL (100 ul/well) can bind Human PD-L1. The ED50 range ≤ 0.5 µg/mL.
Product Name Alternative
Programmed cell death 1 ligand 1 isoform, CD274, B7-H, B7H1, PD-L1, PDCD1L1, PDCD1LG1, PDL1
Expression System
Baculovirus
Antigen Species
Human
Tag
HIgG-His-Tag
Applications
SDS-PAGE, Bioactivity
Concentration
1 mg/mL (determined by absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 95% by SDS-PAGE
Bioactivity
Measured by its binding ability in a functional ELISA with Human PD-1 (CAT# ATGP4027) . The ED50 range ≤ 0.5 µg/mL.
Molecular Weight
52.5 kDa (462aa)
Additionnal Information
References & Citations
Dong H., et al, (1999) Nat. Med. 5:1365-1369.; ; Trabattoni D., et al, (2003) Blood 101:2514-2520.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Immunology
NCBI Accession Number
NP_054862.1
Uniprot Accession Number
Q9NZQ7
Species
Human
N Terminal Sequence
Ala
AA Sequence
Frequently Asked Questions
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