Recombinant canine IFN-gamma/IFNG protein

Ifng, also known as interferon gamma, is a member of the type II interferon family. This protein is a soluble cytokine with antiviral, immunoregulatory and anti-tumor properties and is a potent activator of macrophages. Mutations in this gene are associated with aplastic anemia. Recombinant canine Ifng protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Interferon gamma, IFN-G, IFN-gamma

Expression System

E.coli

Antigen Species

Canine

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Molecular Weight

19.3 kDa (166aa) Confirmed by MALDI-TOF

Additionnal Information

IFN-g, Interferon gamma, IFN-G, IFN-gamma, ATGP3626-10 µg, ATGP3626-20 µg, ATGP3626-50 µg, ATGP3626-100 µg, ATGP3626-250 µg, ATGP3626-500 µg, ATGP3626-1 mg, ATGP3626-10, ATGP3626-20, ATGP3626-50, ATGP3626-100, ATGP3626-250, ATGP3626-500, ATGP3626-1

References & Citations

Barbosa MA., et al. (2011) J comp pathol. 145 (4) :336-44.; ; Stehle ME., et al. (2010) Vet Dermatol. 21 (1) :112-7.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM MES buffer (pH 6.0) containing 20% glycerol, 0.1M NaCl, 1mM EDTA

Scientific Category

Hormones & Cytokines

NCBI Accession Number

NP_001003174

Uniprot Accession Number

P42161

Species

Canine

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSQAMFFKE IENLKEYFNA SNPDVSDGGS LFVDILKKWR EESDKTIIQS QIVSFYLKLF DNFKDNQIIQ RSMDTIKEDM LGKFLNSSTS KREDFLKLIQ IPVNDLQVQR KAINELIKVM NDLSPRSNLR KRKRSQNLFR GRRASK