Recombinant human CD99 protein
CD99, also known as CD99 antigen isoform a, is a type 1 transmembrane glycoprotein and the founding member of the CD99 family of molecules. Cells known to express this protein include fibroblasts, neutrophils, T cells, CD34+ stem cells, monocytes and endothelial cells. Also, it plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Recombinant human CD99, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
CD99 antigen isoform a, CD99, HBA71, MIC2, MIC2X, MIC2Y, MSK5X
Expression System
Baculovirus
Antigen Species
Human
Tag
HIgG-His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 95% by SDS-PAGE
Molecular Weight
37.3 kDa (342aa)
Additionnal Information
References & Citations
Hahn JH., et al, (1997) J. Immunol. 159:2250-2258.; ; Gil M., et al, (2015) Immunol. Lett. 168:329-336.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Immunology
NCBI Accession Number
NP_002405
Uniprot Accession Number
P14209
Species
Human
N Terminal Sequence
Ala
AA Sequence
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