Recombinant human CD99 protein

CD99, also known as CD99 antigen isoform a, is a type 1 transmembrane glycoprotein and the founding member of the CD99 family of molecules. Cells known to express this protein include fibroblasts, neutrophils, T cells, CD34+ stem cells, monocytes and endothelial cells. Also, it plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Recombinant human CD99, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

CD99 antigen isoform a, CD99, HBA71, MIC2, MIC2X, MIC2Y, MSK5X

Expression System

Baculovirus

Antigen Species

Human

Tag

HIgG-His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Molecular Weight

37.3 kDa (342aa)

Additionnal Information

CD99, CD99 antigen isoform a, CD99, HBA71, MIC2, MIC2X, MIC2Y, MSK5X, ATGP3615-10 µg, ATGP3615-20 µg, ATGP3615-50 µg, ATGP3615-100 µg, ATGP3615-250 µg, ATGP3615-500 µg, ATGP3615-1 mg, ATGP3615-10, ATGP3615-20, ATGP3615-50, ATGP3615-100, ATGP3615-250, ATGP3615-500, ATGP3615-1

References & Citations

Hahn JH., et al, (1997) J. Immunol. 159:2250-2258.; ; Gil M., et al, (2015) Immunol. Lett. 168:329-336.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Immunology

NCBI Accession Number

NP_002405

Uniprot Accession Number

P14209

Species

Human

N Terminal Sequence

Ala

AA Sequence

ADPDGGFDLS DALPDNENKK PTAIPKKPSA GDDFDLGDAV VDGENDDPRP PNPPKPMPNP NPNHPSSSGS FSDADLADGV SGGEGKGGSD GGGSHRKEGE EADLEPKSCD KTHTCPPCPA PELLGGPSVF LFPPKPKDTL MISRTPEVTC VVVDVSHEDP EVKFNWYVDG VEVHNAKTKP REEQYNSTYR VVSVLTVLHQ DWLNGKEYKC KVSNKALPAP IEKTISKAKG QPREPQVYTL PPSRDELTKN QVSLTCLVKG FYPSDIAVEW ESNGQPENNY KTTPPVLDSD GSFFLYSKLT VDKSRWQQGN VFSCSVMHEA LHNHYTQKSL SLSPGKHHHH HH