Recombinant human t-Plasminogen Activator/PLAT protein

PLAT, also known as tissue-type plasminogen activator isoform 1, is a protein involved in the breakdown of blood clots. This protein is secreted as a single chain polypeptide precursor which is cleaved in turn by plasmin. Also, active PLAT converts plasminogen to plasmin, a fibrinolytic protease, by hydrolyzing an Arg-Val peptide bond in plasminogen. Recombinant human PLAT protein, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Tissue-type plasminogen activator isoform 1, PLAT, T-PA, TPA

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Molecular Weight

32 kDa (284aa)

Additionnal Information

T-Plasminogen Activator, tPA, Tissue-type plasminogen activator isoform 1, PLAT, T-PA, TPA, ATGP3614-10 µg, ATGP3614-20 µg, ATGP3614-50 µg, ATGP3614-100 µg, ATGP3614-250 µg, ATGP3614-500 µg, ATGP3614-1 mg, ATGP3614-10, ATGP3614-20, ATGP3614-50, ATGP3614-100, ATGP3614-250, ATGP3614-500, ATGP3614-1

References & Citations

Chevilley A., et al, (2015) Front Cell Neurosci. 9:415.; ; Lansley SM., et al, (2015) Am. J. Respir. Cell Mol. Biol. 53:105-112.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 50 mM MES buffer (pH 5.5) containing 10% glycerol, 100 mM NaCl, 5mM CaCl2.

Scientific Category

Cardiovascular

NCBI Accession Number

NP_000921

Uniprot Accession Number

P00750

Species

Human

N Terminal Sequence

Ala

AA Sequence

SYQVICR DEKTQMIYQQ HQSWLRPVLR SNRVEYCWCN SGRAQCHSVP VKSCSEPRCF NGGTCQQALY FSDFVCQCPE GFAGKCCEID TRATCYEDQG ISYRGTWSTA ESGAECTNWN SSALAQKPYS GRRPDAIRLG LGNHNYCRNP DRDSKPWCYV FKAGKYSSEF CSTPACSEGN SDCYFGNGSA YRGTHSLTES GASCLPWNSM ILIGKVYTAQ NPSAQALGLG KHNYCRNPDG DAKPWCHVLK NRRLTWEYCD VPSCSTCGLR QYSQPQFR