Recombinant feline IL-2 (C146S) protein
IL2, also known as interleukin2, is an immunoregulatory lymphokine produced by T-cell in response to antigenic or mitogenic stimulation IL2/IL-2R signaling is required for T-cell proliferation and other fundamental functions which are essential for the immune response. IL2 stimulates growth and differentiation of B-cells, NK cells, lymphokine activated kill cells, monocytes, macrophages and oligodendrocytes. Recombinant feline IL2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Feline IL-2 (C146S) stimulates cell proliferation of the CTLL2 mouse cytotoxic T cells. The ED50 range ≤0.3 ng/mL.
Product Name Alternative
Interleukin-2, IL-2, T-cell growth factor, TCGF
Expression System
E.coli
Antigen Species
Feline
Tag
His-Tag
Applications
SDS-PAGE, Bioactivity
Concentration
0.25 mg/mL (determined by BCA assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 95% by SDS-PAGE
Bioactivity
Measured in a cell proliferation assay using CTLL2 mouse cytotoxic T cells. The ED50 range ≤0.3 ng/mL.
Molecular Weight
17.8 kDa (157aa) Confirmed by MALDI-TOF
Additionnal Information
References & Citations
Taniguchi T., et al. (1983) . Nature. 302 (5906) : 305-10; ; Nedoszytko B., et al. (2016) Postepy Dermatol Alerqol. 33 (6) : 429-434
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 30% glycerol
Scientific Category
Hormones & Cytokines
NCBI Accession Number
NP_001036802
Uniprot Accession Number
Q07885
Species
Feline
AA Sequence
APASSST KETQQQLEQL LLDLRLLLNG VNNPENPKLS RMLTFKFYVP KKATELTHLQ CLVEELKPLE EVLYLAQSKN FHLNHIKELM SNINVTVLKL KGSETRFTCN YDDETATIVE FLNKWITFCQ SIFSTLT
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