Recombinant human TFPI protein

TFPI, also known as tissue factor pathway inhibitor isoform, is the major regulator of tissue factor (TF) -induced coagulation. It is predominantly produced by microvascular endothelial cells, though it is also found in platelets, monocytes, smooth muscle cells, and plasma. It is an anticoagulant protein that inhibits early phases of the procoagulant response. It has been shown to be associated with breast cancer pathogenesis. It consists of a highly negatively charged amino-terminus, three tandemly linked Kunitz domains, and a highly positively charged carboxy-terminus. It exhibits significant homology with human inter-alpha-trypsin inhibitor and bovine basic pancreatic trypsin inhibitor. Recombinant human TFPI, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.