Recombinant mouse NMNAT-1 protein
Nmnat1, also known as nicotinamide/nicotinic acid mononucleotide adenylyltransferase1, is a central enzyme in NAD biosynthesis, catalyzing the condensation of nicotinamide mononucleotide (NMN) of nicotinic acid mononucleotide (NaMN) with the AMP moiety of ATP to form NAD or NaAD. It is widely expressed with high levels in skeletal muscle, heart, liver and kidney. This protein appears to have the ability to protect against axonal degeneration following mechanical or toxic insults. Recombinant mouse Nmnat1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
Mouse
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by absorbance at 280 nm)
Purity
> 95% by SDS-PAGE
Molecular Weight
34.7 kDa (308aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Emanuelli M., et al. (2001) J Biol Chem. 276 (1) :406-12; ; Zhou T., et al. (2002) J Biol Chem. 277 (15) 13148-54.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol, 1mM EDTA
Scientific Category
Neuroscience
NCBI Accession Number
NP_597679
Uniprot Accession Number
Q9EPA7
Species
Mouse
AA Sequence
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