Recombinant human IL-17F protein

IL17F, also known as Interleukin-17F, is a cytokine that shares sequence similarity with IL17. It is expressed in activated CD4+ T-cells and activated monocytes. The biological activities mediated by IL17F are similar to those of IL-17. This protein stimulates production of IL-6, IL-8, G-CSF, and regulates cartilage matrix turnover by increasing matrix release and inhibiting new matrix synthesis. It also inhibits angiogenesis and induces production of IL-2, TGF-beta, and monocyte chemoattractant protein-1 in endothelial cells Recombinant human IL17F protein, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

CANDF6, IL-17F, Interleukin-17F, Interleukin-2, Macrophagleukin-17F, ML-1

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Molecular Weight

16kDa (142aa)

Additionnal Information

CANDF6, IL 17F, IL17F, IL-17F, Interleukin 17F, Interleukin 2, Interleukin17F, Interleukin-17F, Interleukin2, Interleukin-2, Macrophagleukin 17F, Macrophagleukin17F, Macrophagleukin-17F, ML1, ML-1, ATGP3605-10 µg, ATGP3605-20 µg, ATGP3605-50 µg, ATGP3605-100 µg, ATGP3605-250 µg, ATGP3605-500 µg, ATGP3605-1 mg, ATGP3605-010, ATGP3605-020, ATGP3605-050, ATGP3605-100, ATGP3605-250, ATGP3605-500, ATGP3605-01M

References & Citations

Fukuyama T., et al, (2012) J Gastrointestin Liver Dis. 21:243-249.; ; Giles DA., et al, (2016) PLoS One. 11:e0149783.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol, 1mM DTT

Scientific Category

Cardiovascular

NCBI Accession Number

NP_443104

Uniprot Accession Number

Q96PD4

Species

Human

N Terminal Sequence

Ala

AA Sequence

ADPRKIPKVG HTFFQKPESC PPVPGGSMKL DIGIINENQR VSMSRNIESR STSPWNYTVT WDPNRYPSEV VQAQCRNLGC INAQGKEDIS MNSVPIQQET LVVRRKHQGC SVSFQLEKVL VTVGCTCVTP VIHHVQHHHH HH