Recombinant mouse OGG1 protein
Ogg1, also known as N-glycosylase/DNA lyase, is a DNA glycosylase enzyme involved in base excision repair. This protein is the primary enzyme responsible for the excision of 7, 8-dihydro-8-oxoguanine (8-oxoG), a mutagenic base byproduct which occurs as a result of exposure to reactive oxygen species (ROS) . It has a beta lyase activity that nicks DNA 3 to the lesion. Recombinant mouse Ogg1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography
Product Specifications
Product Name Alternative
N-glycosylase/DNA lyase, Mmh, 8-oxoguanine DNA-glycosylase 1, DNA-apurinic or apyrimidinic site lyase, AP lyase
Expression System
E.coli
Antigen Species
Mouse
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by absorbance at 280 nm)
Purity
> 90% by SDS-PAGE
Molecular Weight
41.3 kDa (368aa) Confirmed by MALDI-TOF
Additionnal Information
References & Citations
Seeberg E., et al. (2002) . Nucleic Acids Res. 30 (11) :2349-57.; ; Hodges NJ., et al (2010) . DNA Repair (Amst) . 9 (2) :144-52.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 30% glycerol
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_035087
Uniprot Accession Number
O08760
Species
Mouse
AA Sequence
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