Recombinant human Siglec-5 protein

SIGLEC5, also known as sialic acid-binding Ig-like lectin 5, belongs to immunoglobulin superfamily and SIGLEC family. This protein is composed of 2 Ig-like C2-type domains and 1 Ig-like V-type domain. Also, it is expressed by monocytic/myeloid lineage cells. It is found at high levels in peripheral blood leukocytes, spleen, and at lower levels in lymph node, appendix, pancreas, lung, and thymus. Recombinant human SIGLEC5, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Sialic acid-binding Ig-like lectin 5, SIGLEC5, CD170, CD33L2, OB-BP2, OBBP2, SIGLEC-5

Expression System

Baculovirus

Antigen Species

Human

Tag

HIgG-His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Molecular Weight

74.2 kDa (667aa)

Additionnal Information

Siglec5, Sialic acid-binding Ig-like lectin 5, SIGLEC5, CD170, CD33L2, OB-BP2, OBBP2, SIGLEC-5, ATGP3601-10 µg, ATGP3601-20 µg, ATGP3601-50 µg, ATGP3601-100 µg, ATGP3601-250 µg, ATGP3601-500 µg, ATGP3601-1 mg, ATGP3601-10, ATGP3601-20, ATGP3601-50, ATGP3601-100, ATGP3601-250, ATGP3601-500, ATGP3601-1

References & Citations

Fong JJ., et al, (2015) EMBO J. 34:2775-2788.; ; Ali SR., et al, (2014) J. Exp. Med. 211:1231-1242.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4)

Scientific Category

Immunology

NCBI Accession Number

NP_003821

Uniprot Accession Number

O15389

Species

Human

N Terminal Sequence

Ala

AA Sequence

EKPVYEL QVQKSVTVQE GLCVLVPCSF SYPWRSWYSS PPLYVYWFRD GEIPYYAEVV ATNNPDRRVK PETQGRFRLL GDVQKKNCSL SIGDARMEDT GSYFFRVERG RDVKYSYQQN KLNLEVTALI EKPDIHFLEP LESGRPTRLS CSLPGSCEAG PPLTFSWTGN ALSPLDPETT RSSELTLTPR PEDHGTNLTC QMKRQGAQVT TERTVQLNVS YAPQTITIFR NGIALEILQN TSYLPVLEGQ ALRLLCDAPS NPPAHLSWFQ GSPALNATPI SNTGILELRR VRSAEEGGFT CRAQHPLGFL QIFLNLSVYS LPQLLGPSCS WEAEGLHCRC SFRARPAPSL CWRLEEKPLE GNSSQGSFKV NSSSAGPWAN SSLILHGGLS SDLKVSCKAW NIYGSQSGSV LLLQGRSNLG TGVVPAAL