Recombinant human B7-H2 protein

ICOSLG, also known as ICOS ligand isoform a, is a number of the B7 family of co-stimulatory molecules related to B7-1 and B7-2. This protein is a transmembrane glycoprotein with extracellular IgV and IgC domains, and binds to ICOS on activated T cells. Its dependent signaling may play an active role in a proliferative response rather than in cytokine and chemokine production. Recombinant human ICOSLG, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

ICOS ligand isoform a, ICOSLG, B7-H2, B7H2, B7RP-1, B7RP1, CD275, GL50, ICOS-L, ICOSL, LICOS

Expression System

Baculovirus

Antigen Species

Human

Tag

HIgG-His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Molecular Weight

53.7 kDa (480aa)

Additionnal Information

B7-H2, ICOS ligand isoform a, ICOSLG, B7-H2, B7H2, B7RP-1, B7RP1, CD275, GL50, ICOS-L, ICOSL, LICOS, ATGP3600-10 µg, ATGP3600-20 µg, ATGP3600-50 µg, ATGP3600-100 µg, ATGP3600-250 µg, ATGP3600-500 µg, ATGP3600-1 mg, ATGP3600-10, ATGP3600-20, ATGP3600-50, ATGP3600-100, ATGP3600-250, ATGP3600-500, ATGP3600-1

References & Citations

Maazi H., et al, (2015) Immunity 42:538-551.; ; Shen C., et al, (2014) Clin. Exp. Allergy 44:831-841.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Immunology

NCBI Accession Number

NP_056074.1

Uniprot Accession Number

O75144

Species

Human

N Terminal Sequence

Ala

AA Sequence

DTQEKEV RAMVGSDVEL SCACPEGSRF DLNDVYVYWQ TSESKTVVTY HIPQNSSLEN VDSRYRNRAL MSPAGMLRGD FSLRLFNVTP QDEQKFHCLV LSQSLGFQEV LSVEVTLHVA ANFSVPVVSA PHSPSQDELT FTCTSINGYP RPNVYWINKT DNSLLDQALQ NDTVFLNMRG LYDVVSVLRI ARTPSVNIGC CIENVLLQQN LTVGSQTGND IGERDKITEN PVSTGEKNAA T