Recombinant human 14-3-3 beta/alpha protein

YWHAB, also known as 14-3-3 protein beta/alpha, is 14-3-3 family plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, beta, gamma, epsilon, sigma, zeta, tau and eta that have been identified in mammals. The 14-3-3 beta, a subtype of the 14-3-3 proteins, was found in B Cells, brain and liver etc. This 14-3-3 beta has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Recombinant human YWHAB, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

YWHAB, YWHAA, Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein beta/alpha, Protein 1054, Protein kinase C inhibitor protein 1, KCIP-1, 14-3-3 protein beta/alpha N-terminally processed, GW128, HEL-S-1, HS1, KCIP-1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by absorbance at 280 nm)

Purity

> 90% by SDS-PAGE

Molecular Weight

30.6 kDa (270aa) confirmed by MALDI-TOF

Additionnal Information

1433 b, 1433 beta, 14-3-3 protein beta/alpha, GW128, HEL-S-1, HS1, KCIP-1, YWHAA, ATGP3590-10 µg, ATGP3590-20 µg, ATGP3590-50 µg, ATGP3590-100 µg, ATGP3590-250 µg, ATGP3590-500 µg, ATGP3590-1 mg, ATGP3590-10, ATGP3590-20, ATGP3590-50, ATGP3590-100, ATGP3590-250, ATGP3590-500, ATGP3590-1

References & Citations

Rodriguez LG., et al. (2005) J Cell Physiol. 202 (1) :285-94.; ; Mils V., et al. (2000) Oncogene. 19 (10) :1257-65.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Signal Transduction

NCBI Accession Number

NP_003395

Uniprot Accession Number

P31946

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSHMTMDKS ELVQKAKLAE QAERYDDMAA AMKAVTEQGH ELSNEERNLL SVAYKNVVGA RRSSWRVISS IEQKTERNEK KQQMGKEYRE KIEAELQDIC NDVLELLDKY LIPNATQPES KVFYLKMKGD YFRYLSEVAS GDNKQTTVSN SQQAYQEAFE ISKKEMQPTH PIRLGLALNF SVFYYEILNS PEKACSLAKT AFDEAIAELD TLNEESYKDS TLIMQLLRDN LTLWTSENQG DEGDAGEGEN