Recombinant mouse Nicotinamide N-Methyltransferase/NNMT protein

Nnmt, also known as Nicotinamide N-methyltransferase isoform1, is the family of transferases, specifically those transferring one-carbon group methyltransferases. It is predominantly expressed in the liver, and a lower expression is seen in the kidney, lung, skeletal muscle, placenta and heart. Nnmt catalyzes the N-methylation of nicotinamide and other pyridines to form pyridinium ions. This activity is important for biotransformation of many drugs and xenobiotic compounds. Recombinant mouse Nnmt, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Nicotinamide N-methyltransferase isoform1

Expression System

E.coli

Antigen Species

Mouse

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by absorbance at 280 nm)

Purity

> 90% by SDS-PAGE

Molecular Weight

32.1 kDa (288aa) confirmed by MALDI-TOF

Additionnal Information

Nnmt, Nicotinamide N-methyltransferase isoform1, ATGP3589-10 µg, ATGP3589-20 µg, ATGP3589-50 µg, ATGP3589-100 µg, ATGP3589-250 µg, ATGP3589-500 µg, ATGP3589-1 mg, ATGP3589-10, ATGP3589-20, ATGP3589-50, ATGP3589-100, ATGP3589-250, ATGP3589-500, ATGP3589-1

References & Citations

Parsons RB., et al. (2002) . J Neuropathol Exp Neurol. 61 (2) :111-24.; ; Smith ML., et al. (1998) . Biochim Biophys Acta. 1442 (2-3) :238-44.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_035054

Uniprot Accession Number

O55239

Species

Mouse

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSHMESGFT SKDTYLSHFN PRDYLEKYYS FGSRHCAENE ILRHLLKNLF KIFCLGAVKG ELLIDIGSGP TIYQLLSACE SFTEIIVSDY TDQNLWELQK WLKKEPGAFD WSPVVTYVCD LEGNRMKGPE KEEKLRRAIK QVLKCDVTQS QPLGGVSLPP ADCLLSTLCL DAACPDLPAY RTALRNLGSL LKPGGFLVMV DALKSSYYMI GEQKFSSLPL GWETVRDAVE EAGYTIEQFE VISQNYSSTT SNNEGLFSLV GRKPGRSE