Recombinant human 14-3-3 epsilon protein

YWHAE, also known as 14-3-3 protein epsilon, is adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, beta, gamma, epsilon, sigma, zeta, tau and eta that have been identified in mammals. The 14-3-3 epsilon, a subtype of the 14-3-3 family of proteins, was thought to be brain and neuron-specific. It has been shown to interact with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. Recombinant human YWHAE, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

YWHAE, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein epsilon, 14-3-3E, HEL2, KCIP-1, MDCR, MDS

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

31.3 kDa (275aa) Confirmed by MALDI-TOF

Additionnal Information

1433 epsilon, 14-3-3 protein epsilon, 14-3-3E, HEL2, KCIP-1, MDCR, MDS, ATGP3587-10 µg, ATGP3587-20 µg, ATGP3587-50 µg, ATGP3587-100 µg, ATGP3587-250 µg, ATGP3587-500 µg, ATGP3587-1 mg, ATGP3587-10, ATGP3587-20, ATGP3587-50, ATGP3587-100, ATGP3587-250, ATGP3587-500, ATGP3587-1

References & Citations

Oriente F, et al. (2005) J Biol Chem. 280 (49) :40642-9. ; ; Conklin DS, et al. (1995) Proc Natl Acad Sci USA. 92 (17) :7892-6

Other References

Wu JS, et al. Ligand-activated peroxisome proliferator-activated receptor-gamma protects against ischemic cerebral infarction and neuronal apoptosis by 14-3-3 epsilon upregulation. (Circulation. 2009) {https://pubmed.ncbi.nlm.nih.gov/19221220/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 1mM DTT, 10% glycerol

Scientific Category

Signal Transduction

NCBI Accession Number

NP_006752

Uniprot Accession Number

P62258

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MDDREDLVYQ AKLAEQAERY DEMVESMKKV AGMDVELTVE ERNLLSVAYK NVIGARRASW RIISSIEQKE ENKGGEDKLK MIREYRQMVE TELKLICCDI LDVLDKHLIP AANTGESKVF YYKMKGDYHR YLAEFATGND RKEAAENSLV AYKAASDIAM TELPPTHPIR LGLALNFSVF YYEILNSPDR ACRLAKAAFD DAIAELDTLS EESYKDSTLI MQLLRDNLTL WTSDMQGDGE EQNKEALQDV EDENQ