Recombinant human GDNFR alpha-3/GFRA3 protein

GFRA3, also known as GDNF family receptor alpha-3, is a plasma membrane glycoprotein. This protein is one of the four GDNF receptors, all of which are glycosylphosphoinositol (GPI) -linked, contain three conserved cysteine repeats, and involved survival of neurons. Also, it promotes the formation of a physical complex between GFRA/GDNFRa and the orphan tyrosin kinase receptor Ret, thereby inducing its tyrosine phosphorylation. Recombinant human GFRA3, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

GDNF family receptor alpha-3, GFRA3, GDNFR3, GFRalpha3

Expression System

Baculovirus

Antigen Species

Human

Tag

HIgG-His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 85% by SDS-PAGE

Molecular Weight

65.5 kDa (585aa)

Additionnal Information

GFR a3, GDNF family receptor alpha-3, GFRA3, GDNFR3, GFRalpha3, ATGP3585-10 µg, ATGP3585-20 µg, ATGP3585-50 µg, ATGP3585-100 µg, ATGP3585-250 µg, ATGP3585-500 µg, ATGP3585-1 mg, ATGP3585-10, ATGP3585-20, ATGP3585-50, ATGP3585-100, ATGP3585-250, ATGP3585-500, ATGP3585-1

References & Citations

Wu ZS., et al, (2013) BMC Cancer 13:34.; ; Baloh RH., et al, (1998) Neuron 21:1291-1302.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Neuroscience

NCBI Accession Number

NP_001487

Uniprot Accession Number

O60609

Species

Human

N Terminal Sequence

Ala

AA Sequence

ADPDPLPTES RLMNSCLQAR RKCQADPTCS AAYHHLDSCT SSISTPLPSE EPSVPADCLE AAQQLRNSSL IGCMCHRRMK NQVACLDIYW TVHRARSLGN YELDVSPYED TVTSKPWKMN LSKLNMLKPD SDLCLKFAML CTLNDKCDRL RKAYGEACSG PHCQRHVCLR QLLTFFEKAA EPHAQGLLLC PCAPNDRGCG ERRRNTIAPN CALPPVAPNC LELRRLCFSD PLCRSRLVDF QTHCHPMDIL GTCATEQSRC LRAYLGLIGT AMTPNFVSNV NTSVALSCTC RGSGNLQEEC EMLEGFFSHN PCLTEAIAAK MRFHSQLFSQ DWPHPTFAVM AHQNENLEPK SCDKTHTCPP CPAPELLGGP SVFLFPPKPK DTLMISRTPE VTCVVVDVSH EDPEVKFNWY VDGVEVHNAK TKPREEQYNS TYRVVSVLTV LHQDWLNGKE YKCKVSNKAL PAPIEKTISK AKGQPREPQV YTLPPSRDEL TKNQVSLTCL VKGFYPSDIA VEWESNGQPE NNYKTTPPVL DSDGSFFLYS KLTVDKSRWQ QGNVFSCSVM HEALHNHYTQ KSLSLSPGKH HHHHH