Recombinant human Glypican 3 protein

GPC3, also known as glypican-3 isoform 2, is a member of the glypican family. It belongs to the glypican family and is highly expressed in lung, liver, and kidney. Also, it has tissue dependent. In some tissues, it acts as a tumor suppressor gene and an oncofetal protein. This protein is currently regarded as a tumor marker and potential target for immunotherapy. Recombinant human GPC3, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Glypican-3 isoform 2, GPC3, DGSX, GTR2-2, MXR7, OCI-5, SDYS, SGB, SGBS, SGBS1

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Molecular Weight

61.8 kDa (544aa)

Additionnal Information

Glypican3, Glypican-3 isoform 2, GPC3, DGSX, GTR2-2, MXR7, OCI-5, SDYS, SGB, SGBS, SGBS1, ATGP3584-10 µg, ATGP3584-20 µg, ATGP3584-50 µg, ATGP3584-100 µg, ATGP3584-250 µg, ATGP3584-500 µg, ATGP3584-1 mg, ATGP3584-10, ATGP3584-20, ATGP3584-50, ATGP3584-100, ATGP3584-250, ATGP3584-500, ATGP3584-1

References & Citations

Feng J., et al, (2016) PLoS ONE 11:E0151501.; ; Davoodi J., et al, (2007) Proteomics 7:2300-2310.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Stem Cell

NCBI Accession Number

NP_004475

Uniprot Accession Number

P51654

Species

Human

N Terminal Sequence

Ala

AA Sequence

ADPQPPPPPP DATCHQVRSF FQRLQPGLKW VPETPVPGSD LQVCLPKGPT CCSRKMEEKY QLTARLNMEQ LLQSASMELK FLIIQNAAVF QEAFEIVVRH AKNYTNAMFK NNYPSLTPQA FEFVGEFFTD VSLYILGSDI NVDDMVNELF DSLFPVIYTQ LMNPGLPDSA LDINECLRGA RRDLKVFGNF PKLIMTQVSK SLQVTRIFLQ ALNLGIEVIN TTDHLKFSKD CGRMLTRMWY CSYCQGLMMV KPCGGYCNVV MQGCMAGVVE IDKYWREYIL SLEELVNGMY RIYDMENVLL GLFSTIHDSI QYVQKNAGKL TTTIGKLCAH SQQRQYRSAY YPEDLFIDKK VLKVAHVEHE ETLSSRRREL IQKLKSFISF YSALPGYICS HSPVAENDTL CWNGQELVER YSQKAARNGM KNQFNLHELK MKGPEPVVSQ IIDKLKHINQ LLRTMSMPKG RVLDKNLDEE GFESGDCGDD EDECIGGSGD GMIKVKNQLR FLAELAYDLD VDDAPGNSQQ ATPKDNEIST FHNLGNVHHH HHHH