Recombinant human DPH2 protein

DPH2, also known as diphthamide biosynthesis protein 2, is a homodimer and each of its monomers can bind a [4Fe-4S] cluster. It is the target of ADP ribosylating diphtheria toxin (DT) and Pseudomonas exotoxin A (PE) . It was identified by its ability to complement a diphthamide mutant strain, and thus functions in diphthamide biosynthesis. Its loss pre-activates NF-kB and death receptor pathways and renders MCF7 cells hypersensitive to tumor necrosis factor. Recombinant human DPH2, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Diphthamide biosynthesis protein 2, DPH2, DPH2L2

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 85% by SDS-PAGE

Molecular Weight

53.1 kDa (497aa)

Additionnal Information

DPH2, Diphthamide biosynthesis protein 2, DPH2, DPH2L2, ATGP3582-10 µg, ATGP3582-20 µg, ATGP3582-50 µg, ATGP3582-100 µg, ATGP3582-250 µg, ATGP3582-500 µg, ATGP3582-1 mg, ATGP3582-10, ATGP3582-20, ATGP3582-50, ATGP3582-100, ATGP3582-250, ATGP3582-500, ATGP3582-1