Recombinant renilla reniformis Luciferase protein
LUC, also known as luciferase. LUC catalyzes the oxidative decarboxylation of coelenterazine in the presence of dissolvedoxygen to yield oxyluciferin, CO2, and blue light. In vivo, the excited state luciferin-luciferase complex undergoes the process of noradiative energy transfer to an accessory protein, green fluorescent protein, which results in green bioluminescence. In vitro, it emits blue light in the absence of any green fluorescent protein. Recombinant renilla reniformis LUC, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Specific activity is >1x10^9 light units/mg. One luciferase enzyme units will produce one Relative Light Unit (RLU) at pH7.5 at 25C.
Product Name Alternative
Luciferase
Expression System
E.coli
Antigen Species
Renilla reniformis
Tag
His-Tag
Applications
SDS-PAGE, Enzyme Activity
Concentration
0.5 mg/mL (determined by Bradford assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 95% by SDS-PAGE
Bioactivity
Specific activity is >1X10^9 lightunit/mg. One luciferase enzymeunit will produce one Relative Light Unit (RLU) at pH7.5 at 25C.
Molecular Weight
38.5 kDa (335aa) Confirmed by MALDI-TOF
Additionnal Information
References & Citations
Lorenz WW., et al. (1991) Proc Natl Acad Sci U S A. 88 (10) :4438-42.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol
Scientific Category
Enzymes & Proteases
NCBI Accession Number
AAA29804.1
Uniprot Accession Number
P27652
Species
Renilla reniformis
AA Sequence
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