Recombinant human PKA C-alpha/PRKACA protein

PRKACA, also known as cAMP-dependent protein kinase catalytic subunit alpha isoform Calpha1, is a member of the Ser/Thr protein kinase family. It is responsible for phosphorylating other proteins and substrates, changing their activity. This protein is a member of the AGC kinase family, and contributes to the control of cellular processes that include glucose metabolism, cell division, and contextual memory. Recombinant human PRKACA protein, fused to GST-tag at N-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

CAMP-dependent protein kinase catalytic subunit alpha isoform Calpha1, PRKACA, PKACA, PPNAD4

Expression System

Baculovirus

Antigen Species

Human

Tag

GST-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 85% by SDS-PAGE

Molecular Weight

67 kDa (578aa)

Additionnal Information

PKA C a, cAMP-dependent protein kinase catalytic subunit alpha isoform Calpha1, PRKACA, PKACA, PPNAD4, ATGP3580-10 µg, ATGP3580-20 µg, ATGP3580-50 µg, ATGP3580-100 µg, ATGP3580-250 µg, ATGP3580-500 µg, ATGP3580-1 mg, ATGP3580-10, ATGP3580-20, ATGP3580-50, ATGP3580-100, ATGP3580-250, ATGP3580-500, ATGP3580-1

References & Citations

Moody SE. et al., (2015) Oncogene. 34: 2061-2071.; ; Nanba K. et al., (2016) Eur J Endocrinol. 175: K1-6.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Signal Transduction

NCBI Accession Number

NP_002721

Uniprot Accession Number

P17612

Species

Human

N Terminal Sequence

Met

AA Sequence

MGN AAAAKKGSEQ ESVKEFLAKA KEDFLKKWES PAQNTAHLDQ FERIKTLGTG SFGRVMLVKH KETGNHYAMK ILDKQKVVKL KQIEHTLNEK RILQAVNFPF LVKLEFSFKD NSNLYMVMEY VPGGEMFSHL RRIGRFSEPH ARFYAAQIVL TFEYLHSLDL IYRDLKPENL LIDQQGYIQV TDFGFAKRVK GRTWTLCGTP EYLAPEIILS KGYNKAVDWW ALGVLIYEMA AGYPPFFADQ PIQIYEKIVS GKVRFPSHFS SDLKDLLRNL LQVDLTKRFG NLKNGVNDIK NHKWFATTDW IAIYQRKVEA PFIPKFKGPG DTSNFDDYEE EEIRVSINEK CGKEFSEF