Recombinant rat GDNFR alpha-1/GFRA1 protein

Gfra1, also known as GDNF family receptor alpha-1, is a member of the GDNF receptor family. It is a glycosyl-phosphatidylinositol (GPI) -linked cell surface receptor for both Glial cell line-derived growth factor (GDNF), neurturin (NTN), and mediates activation of the RET tyrosine kinase receptor. This protein is a potent survival factor for central and peripheral neurons, and is essential for the development of kidneys and the enteric nervous system. Recombinant rat Gfra1 protein, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

GDNF family receptor alpha-1, Gfra1, GFR-alpha-1, RET ligand 1, TGF-beta-related neurotrophic factor receptor 1, Retl1, Trnr1

Expression System

Baculovirus

Antigen Species

Rat

Tag

HIgG-His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 85% by SDS-PAGE

Molecular Weight

72.3 kDa (645aa)

Additionnal Information

GDNF family receptor alpha 1, GDNF family receptor alpha-1, GFR a1, GFR alpha 1, Gfra 1, Gfra1, Gfra-1, GFR-alpha-1, RET ligand 1, Retl 1, Retl1, TGF beta related neurotrophic factor receptor 1, TGF-beta-related neurotrophic factor receptor 1, Trnr 1, Trnr1, ATGP3579-10 µg, ATGP3579-20 µg, ATGP3579-50 µg, ATGP3579-100 µg, ATGP3579-250 µg, ATGP3579-500 µg, ATGP3579-1 mg, ATGP3579-010, ATGP3579-020, ATGP3579-050, ATGP3579-100, ATGP3579-250, ATGP3579-500, ATGP3579-01M

References & Citations

Gassei K. et al., (2009) Cell Tissue Res. 337:177-183.; ; Irala D. et al., (2016) Development. 143: 4224-4235.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Neuroscience

NCBI Accession Number

NP_037091

Uniprot Accession Number

Q62997

Species

Rat

N Terminal Sequence

Asp25

AA Sequence

DRLDCVKASD QCLKEQSCST KYRTLRQCVA GKETNFSLTS GLEAKDECRS AMEALKQKSL YNCRCKRGMK KEKNCLRIYW SMYQSLQGND LLEDSPYEPV NSRLSDIFRA VPFISDVFQQ VEHISKGNNC LDAAKACNLD DTCKKYRSAY ITPCTTSMSN EVCNRRKCHK ALRQFFDKVP AKHSYGMLFC SCRDIACTER RRQTIVPVCS YEERERPNCL SLQDSCKTNY ICRSRLADFF TNCQPESRSV SNCLKENYAD CLLAYSGLIG TVMTPNYVDS SSLSVAPWCD CSNSGNDLED CLKFLNFFKD NTCLKNAIQA FGNGSDVTMW QPAPPVQTTT ATTTTAFRVK NKPLGPAGSE NEIPTHVLPP CANLQAQKLK SNVSGSTHLC LSDSDFGKDG LAGASSLEPK SCDKTHTCPP CPAPELLGGP SVFLFPPKPK DTLMISRTPE VTCVVVDVSH EDPEVKFNWY VDGVEVHNAK TKPREEQYNS TYRVVSVLTV LHQDWLNGKE YKCKVSNKAL PAPIEKTISK AKGQPREPQV YTLPPSRDEL TKNQVSLTCL VKGFYPSDIA VEWESNGQPE NNYKTTPPVL DSDGSFFLYS KLTVDKSRWQ QGNVFSCSVM HEALHNHYTQ KSLSLSPGKH HHHHH